1.For some materials, we could supply free Samples for testing at first..
2.MOQ: 1g or 5g
3.More than 3000 kinds of materials in stock.
4.More than 11+ manufacturing experience.
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10. Our Q&C team will inspect every product quality strictly before delivery.
What is CAS 3483-12-3 DL-Dithiothreitol Powder?
Product Name: |
DL-Dithiothreitol |
CAS: |
3483-12-3 |
MF: |
C4H10O2S2 |
MW: |
154.25 |
EINECS: |
222-468-7 |
Dithiothreitol, also known as dimercaptothreitol (DTT for short), is a common reducing agent with strong reducibility, which is largely due to the conformational stability of its oxidized six-membered ring (containing disulfide bonds).
1.Molecular biology
2.Quantitative reduction of disulfides; DTT can effectively reduce protein disulfide bonds in the sample buffer before SDS-PAGE; DTT can also be used to reduce the disulfide bonds of the cross-linking agent N,N′-bis(acryloyl)cystamine Bridge to decompose polyacrylamide gel matrix; in protein sequence analysis, to remove disulfide linkages; to investigate increased and dynamic reduction reactions; to prevent the extraction of cholecystokinin from brain and duodenal tissue When the oxidation reaction occurs.
3. DL-1,4-Dithiothreitol is as a reducing agent and deprotecting agent for thiolated DNA. The thiolated DNA terminal sulfur atom tends to form a dimer in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as the immobilization of DNA in biosensors); while adding DTT to the DNA solution and removing it after a period of reaction, the dimerization of DNA can be reduced.
4. DL-1,4-Dithiothreitolis also often used for the reduction of disulfide bonds in proteins and can be used to prevent intramolecular or intermolecular disulfide bonds of proteins formed between cysteines in proteins. However, DTT often cannot reduce the disulfide bonds embedded in the protein structure (solvent unreachable). The reduction of such disulfide bonds often requires denaturation of the protein (heating at high temperature or adding a denaturing agent such as 6M guanidine hydrochloride, 8M urea or 1% SDS). On the contrary, according to the difference in the rate of reduction of disulfide bonds in the presence of DTT, the degree of embedding can be judged.
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Q&A
1.How could l get a sample?
Before we received the first order, please afford the sample cost and express fee. We will return the sample cost back to you within your first order.
2.Can you make designs for us?
We have a professional design team to help our customers do design work.Both OEM and ODM orders are accepted.
3.Whether you could make our brand on your products?
Yes. We can print your Logo on both the products and the packages if you can meet our MOQ.
4.How can you provide us high quality products?
We have a professional QC team, every product is shipped after strictly examination.
5.What is your lead time?
7-15days, We will update you eact two-three days!
6.What is your payment?
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