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What is CAS 1132-61-2 MOPS Powder?
| Product Name: |
MOPS |
| CAS: |
1132-61-2 |
| MF: |
C7H15NO4S |
| MW: |
209.26 |
| EINECS: |
214-478-5 |
3-Morpholinopropanesulonic Acid is a chemical substance, also known as MOPS, with the molecular formula C7H15NO4S. MOPS is a zwitterionic buffering reagent commonly used in biochemistry and molecular biology, especially in applications that require control of solution pH. Its structure is similar to 2- (N-morpholine) ethanesulfonic acid (MES), both of which are specially developed "Good" buffer components.
Function of MOPS:
Biological buffer: MOPS has buffering ability in the pH range of 6.5 to 7.9 and is widely used in biological and biochemical research due to its inert properties.
Cell culture: MOPS buffer can maintain the pH value of mammalian cell culture medium, which helps with cell growth and maintains the physiological environment.
Electrophoresis experiment: MOPS is used for denaturing gel electrophoresis of RNA to keep pH stable during electrophoresis.
Protein research: MOPS can interact with bovine serum albumin and stabilize the protein, playing an important role in protein purification and research.
Microbial culture medium: As a buffering agent in microbial growth culture medium, it helps promote the growth and reproduction of microorganisms.
Application of MOPS:
Cell culture and molecular biology experiments: MOPS, due to its excellent buffering capacity, can help maintain a stable pH environment, which is necessary for many biochemical reactions. It is widely used in cell culture and molecular biology experiments to ensure the accuracy and reliability of the experiments.
Analytical chemistry experiments: MOPS is also used in some analytical chemistry experiments, such as ion chromatography and electrophoresis experiments, to help maintain the pH value of the experimental environment and ensure the accuracy of experimental results.
Biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits: MOPS is also used as a biological buffer in biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits, which is crucial for maintaining the performance and accuracy of the kits.
Organic synthesis and medicinal chemistry: Although MOPS is mainly used as a biological buffering agent, the amide units in its structure also make it applicable in organic synthesis and medicinal chemistry. For example, morpholin-3-one, as an organic synthesis reagent and medicinal chemical intermediate, can be used for the preparation of drug molecules, such as the synthesis of rivaroxaban.
Protein Purification and Chromatography: MOPS is commonly used in protein purification and chromatography, especially in cell culture media, electrophoresis buffer, and protein purification processes, to assist in the separation and purification of proteins.
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